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Promega pgem-t-easy ligation kit
Pgem T Easy Ligation Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgem-t+easy+ligation+kit/pgem+t+easy/pm36721160-239-16-21
Average 90 stars, based on 1 article reviews
pgem-t-easy ligation kit - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Purification:

Article Title: Development and validation of a universal S-segment-based real-time RT-PCR assay for the detection of Simbu serogroup viruses.
Article Snippet: Simbu serogroup viruses induce acute clinical diseases and abnormal courses of pregnancies in livestock.. In Israel, two members of this serogroup, namely Akabane virus (AKAV) and Shuni virus (SHUV), were recently detected and, in Europe, Schmallenberg virus (SBV) poses a threat to ruminants.. To address this emerging problem, a universal S-segment-based real-time RT-PCR assay (Uni-S) for the detection of Simbu serogroup viruses was established, which, additionally, enabled species identification of the detected viruses by subsequent sequencing.

Article Title: Divergent expression patterns of miR164 and CUP-SHAPED COTYLEDON genes in palms and other monocots: implication for the evolution of meristem function in angiosperms.
Article Snippet: The PCR products were cloned using a pGEM-T easy ligation kit (Promega, France) with Escherichia coli JM109 competent cells (Promega) and sequenced by Beckman Coulter Genomics (UK).

Article Title: Genetic mutations in sodium channel domain II and carboxylesterase genes associated with phenotypic resistance against synthetic pyrethroids by Rhipicephalus (Boophilus) decoloratus ticks in Uganda.
Article Snippet: Please cite this article as: Patrick Vudriko, Rika Umemiya-Shirafuji, James Okwee-Acai, Dickson Stuart Tayebwa, Joseph Byaruhanga, Charoonluk Jirapattharasate, Mingming Liu, Paul Franck Adjou Moumouni, Kozo Fujisaki, Xuenan Xuan, Hiroshi Suzuki , Genetic mutations in sodium channel domain II and carboxylesterase genes associated with phenotypic resistance against synthetic pyrethroids by Rhipicephalus (Boophilus) decoloratus ticks in Uganda, Pesticide Biochemistry and Physiology (2017), doi: 10.1016/ j.pestbp.2017.07.009

Article Title: C190A knockdown mutation in sodium channel domain II of pyrethroid-resistant Rhipicephalus appendiculatus.
Article Snippet: This study investigated the target site mutations in the partial sequence of voltage-sensitive sodium channel (VSSC) domain II of synthetic pyrethroid (SP)-resistant Rhipicephalus appendiculatus.. Genomic DNA was extracted from seven tick populations (two susceptible and five resistant) collected from central, eastern and southwestern Uganda.. The PCR amplicons of the VSSC domain II were cloned and sequenced to determine novel single nucleotide polymorphisms (SNP).

Article Title: Conserved miR164-targeted NAC genes negatively regulate drought resistance in rice
Article Snippet: PCR products were cloned using the pGEM-T easy ligation kit (Promega) with Escherichia coli Top10 competent cells (Invitrogen) and sequenced.

Article Title: Sequence conservation and divergence in miR164C1 and its target, CUC1, in Brassica species
Article Snippet: An emerging aspect of plant development and adaptation studies is microRNA (miRNA)-mediated gene regulation and to understand post-transcriptional gene silencing (PTGS) dynamics.. Studies performed within an evolutionary context, such as specific plant lineages and genome status (e.g. ploidy level) can highlight the selection pressure and constraints guiding the evolutionary trajectory of regulatory modules.. We have investigated conservation and allelic diversity in one such module composed of CUC1 (CUP-SHAPED COTYLEDON 1), a NAC domain family member, and miR164C, within Brassica species.

Article Title: Systems and methods to assess microbiomes and treatments thereof
Article Snippet: One to three microliters of the 20 μl purified product was ligated into pGEM-T Easy vector using the pGEM-T Easy Ligation Kit (Promega) according to the manufacturer's specifications.

Plasmid Preparation:

Article Title: Development and validation of a universal S-segment-based real-time RT-PCR assay for the detection of Simbu serogroup viruses.
Article Snippet: Simbu serogroup viruses induce acute clinical diseases and abnormal courses of pregnancies in livestock.. In Israel, two members of this serogroup, namely Akabane virus (AKAV) and Shuni virus (SHUV), were recently detected and, in Europe, Schmallenberg virus (SBV) poses a threat to ruminants.. To address this emerging problem, a universal S-segment-based real-time RT-PCR assay (Uni-S) for the detection of Simbu serogroup viruses was established, which, additionally, enabled species identification of the detected viruses by subsequent sequencing.

Article Title: Divergent expression patterns of miR164 and CUP-SHAPED COTYLEDON genes in palms and other monocots: implication for the evolution of meristem function in angiosperms.
Article Snippet: The PCR products were cloned using a pGEM-T easy ligation kit (Promega, France) with Escherichia coli JM109 competent cells (Promega) and sequenced by Beckman Coulter Genomics (UK).

Article Title: Genetic mutations in sodium channel domain II and carboxylesterase genes associated with phenotypic resistance against synthetic pyrethroids by Rhipicephalus (Boophilus) decoloratus ticks in Uganda.
Article Snippet: Please cite this article as: Patrick Vudriko, Rika Umemiya-Shirafuji, James Okwee-Acai, Dickson Stuart Tayebwa, Joseph Byaruhanga, Charoonluk Jirapattharasate, Mingming Liu, Paul Franck Adjou Moumouni, Kozo Fujisaki, Xuenan Xuan, Hiroshi Suzuki , Genetic mutations in sodium channel domain II and carboxylesterase genes associated with phenotypic resistance against synthetic pyrethroids by Rhipicephalus (Boophilus) decoloratus ticks in Uganda, Pesticide Biochemistry and Physiology (2017), doi: 10.1016/ j.pestbp.2017.07.009

Article Title: C190A knockdown mutation in sodium channel domain II of pyrethroid-resistant Rhipicephalus appendiculatus.
Article Snippet: This study investigated the target site mutations in the partial sequence of voltage-sensitive sodium channel (VSSC) domain II of synthetic pyrethroid (SP)-resistant Rhipicephalus appendiculatus.. Genomic DNA was extracted from seven tick populations (two susceptible and five resistant) collected from central, eastern and southwestern Uganda.. The PCR amplicons of the VSSC domain II were cloned and sequenced to determine novel single nucleotide polymorphisms (SNP).

Article Title: Conserved miR164-targeted NAC genes negatively regulate drought resistance in rice
Article Snippet: PCR products were cloned using the pGEM-T easy ligation kit (Promega) with Escherichia coli Top10 competent cells (Invitrogen) and sequenced.

Article Title: Sequence conservation and divergence in miR164C1 and its target, CUC1, in Brassica species
Article Snippet: An emerging aspect of plant development and adaptation studies is microRNA (miRNA)-mediated gene regulation and to understand post-transcriptional gene silencing (PTGS) dynamics.. Studies performed within an evolutionary context, such as specific plant lineages and genome status (e.g. ploidy level) can highlight the selection pressure and constraints guiding the evolutionary trajectory of regulatory modules.. We have investigated conservation and allelic diversity in one such module composed of CUC1 (CUP-SHAPED COTYLEDON 1), a NAC domain family member, and miR164C, within Brassica species.

Article Title: Systems and methods to assess microbiomes and treatments thereof
Article Snippet: One to three microliters of the 20 μl purified product was ligated into pGEM-T Easy vector using the pGEM-T Easy Ligation Kit (Promega) according to the manufacturer's specifications.

Ligation:

Article Title: Development and validation of a universal S-segment-based real-time RT-PCR assay for the detection of Simbu serogroup viruses.
Article Snippet: Simbu serogroup viruses induce acute clinical diseases and abnormal courses of pregnancies in livestock.. In Israel, two members of this serogroup, namely Akabane virus (AKAV) and Shuni virus (SHUV), were recently detected and, in Europe, Schmallenberg virus (SBV) poses a threat to ruminants.. To address this emerging problem, a universal S-segment-based real-time RT-PCR assay (Uni-S) for the detection of Simbu serogroup viruses was established, which, additionally, enabled species identification of the detected viruses by subsequent sequencing.

Article Title: Divergent expression patterns of miR164 and CUP-SHAPED COTYLEDON genes in palms and other monocots: implication for the evolution of meristem function in angiosperms.
Article Snippet: The PCR products were cloned using a pGEM-T easy ligation kit (Promega, France) with Escherichia coli JM109 competent cells (Promega) and sequenced by Beckman Coulter Genomics (UK).

Article Title: Genetic mutations in sodium channel domain II and carboxylesterase genes associated with phenotypic resistance against synthetic pyrethroids by Rhipicephalus (Boophilus) decoloratus ticks in Uganda.
Article Snippet: Please cite this article as: Patrick Vudriko, Rika Umemiya-Shirafuji, James Okwee-Acai, Dickson Stuart Tayebwa, Joseph Byaruhanga, Charoonluk Jirapattharasate, Mingming Liu, Paul Franck Adjou Moumouni, Kozo Fujisaki, Xuenan Xuan, Hiroshi Suzuki , Genetic mutations in sodium channel domain II and carboxylesterase genes associated with phenotypic resistance against synthetic pyrethroids by Rhipicephalus (Boophilus) decoloratus ticks in Uganda, Pesticide Biochemistry and Physiology (2017), doi: 10.1016/ j.pestbp.2017.07.009

Article Title: C190A knockdown mutation in sodium channel domain II of pyrethroid-resistant Rhipicephalus appendiculatus.
Article Snippet: This study investigated the target site mutations in the partial sequence of voltage-sensitive sodium channel (VSSC) domain II of synthetic pyrethroid (SP)-resistant Rhipicephalus appendiculatus.. Genomic DNA was extracted from seven tick populations (two susceptible and five resistant) collected from central, eastern and southwestern Uganda.. The PCR amplicons of the VSSC domain II were cloned and sequenced to determine novel single nucleotide polymorphisms (SNP).

Article Title: Conserved miR164-targeted NAC genes negatively regulate drought resistance in rice
Article Snippet: PCR products were cloned using the pGEM-T easy ligation kit (Promega) with Escherichia coli Top10 competent cells (Invitrogen) and sequenced.

Article Title: Sequence conservation and divergence in miR164C1 and its target, CUC1, in Brassica species
Article Snippet: An emerging aspect of plant development and adaptation studies is microRNA (miRNA)-mediated gene regulation and to understand post-transcriptional gene silencing (PTGS) dynamics.. Studies performed within an evolutionary context, such as specific plant lineages and genome status (e.g. ploidy level) can highlight the selection pressure and constraints guiding the evolutionary trajectory of regulatory modules.. We have investigated conservation and allelic diversity in one such module composed of CUC1 (CUP-SHAPED COTYLEDON 1), a NAC domain family member, and miR164C, within Brassica species.

Article Title: Systems and methods to assess microbiomes and treatments thereof
Article Snippet: One to three microliters of the 20 μl purified product was ligated into pGEM-T Easy vector using the pGEM-T Easy Ligation Kit (Promega) according to the manufacturer's specifications.

TA Cloning:

Article Title: Development and validation of a universal S-segment-based real-time RT-PCR assay for the detection of Simbu serogroup viruses.
Article Snippet: Simbu serogroup viruses induce acute clinical diseases and abnormal courses of pregnancies in livestock.. In Israel, two members of this serogroup, namely Akabane virus (AKAV) and Shuni virus (SHUV), were recently detected and, in Europe, Schmallenberg virus (SBV) poses a threat to ruminants.. To address this emerging problem, a universal S-segment-based real-time RT-PCR assay (Uni-S) for the detection of Simbu serogroup viruses was established, which, additionally, enabled species identification of the detected viruses by subsequent sequencing.

Article Title: Divergent expression patterns of miR164 and CUP-SHAPED COTYLEDON genes in palms and other monocots: implication for the evolution of meristem function in angiosperms.
Article Snippet: The PCR products were cloned using a pGEM-T easy ligation kit (Promega, France) with Escherichia coli JM109 competent cells (Promega) and sequenced by Beckman Coulter Genomics (UK).

Article Title: Genetic mutations in sodium channel domain II and carboxylesterase genes associated with phenotypic resistance against synthetic pyrethroids by Rhipicephalus (Boophilus) decoloratus ticks in Uganda.
Article Snippet: Please cite this article as: Patrick Vudriko, Rika Umemiya-Shirafuji, James Okwee-Acai, Dickson Stuart Tayebwa, Joseph Byaruhanga, Charoonluk Jirapattharasate, Mingming Liu, Paul Franck Adjou Moumouni, Kozo Fujisaki, Xuenan Xuan, Hiroshi Suzuki , Genetic mutations in sodium channel domain II and carboxylesterase genes associated with phenotypic resistance against synthetic pyrethroids by Rhipicephalus (Boophilus) decoloratus ticks in Uganda, Pesticide Biochemistry and Physiology (2017), doi: 10.1016/ j.pestbp.2017.07.009

Article Title: C190A knockdown mutation in sodium channel domain II of pyrethroid-resistant Rhipicephalus appendiculatus.
Article Snippet: This study investigated the target site mutations in the partial sequence of voltage-sensitive sodium channel (VSSC) domain II of synthetic pyrethroid (SP)-resistant Rhipicephalus appendiculatus.. Genomic DNA was extracted from seven tick populations (two susceptible and five resistant) collected from central, eastern and southwestern Uganda.. The PCR amplicons of the VSSC domain II were cloned and sequenced to determine novel single nucleotide polymorphisms (SNP).

Article Title: Conserved miR164-targeted NAC genes negatively regulate drought resistance in rice
Article Snippet: PCR products were cloned using the pGEM-T easy ligation kit (Promega) with Escherichia coli Top10 competent cells (Invitrogen) and sequenced.

Article Title: Sequence conservation and divergence in miR164C1 and its target, CUC1, in Brassica species
Article Snippet: An emerging aspect of plant development and adaptation studies is microRNA (miRNA)-mediated gene regulation and to understand post-transcriptional gene silencing (PTGS) dynamics.. Studies performed within an evolutionary context, such as specific plant lineages and genome status (e.g. ploidy level) can highlight the selection pressure and constraints guiding the evolutionary trajectory of regulatory modules.. We have investigated conservation and allelic diversity in one such module composed of CUC1 (CUP-SHAPED COTYLEDON 1), a NAC domain family member, and miR164C, within Brassica species.

Article Title: Systems and methods to assess microbiomes and treatments thereof
Article Snippet: One to three microliters of the 20 μl purified product was ligated into pGEM-T Easy vector using the pGEM-T Easy Ligation Kit (Promega) according to the manufacturer's specifications.

Polymerase Chain Reaction:

Article Title: Development and validation of a universal S-segment-based real-time RT-PCR assay for the detection of Simbu serogroup viruses.
Article Snippet: Simbu serogroup viruses induce acute clinical diseases and abnormal courses of pregnancies in livestock.. In Israel, two members of this serogroup, namely Akabane virus (AKAV) and Shuni virus (SHUV), were recently detected and, in Europe, Schmallenberg virus (SBV) poses a threat to ruminants.. To address this emerging problem, a universal S-segment-based real-time RT-PCR assay (Uni-S) for the detection of Simbu serogroup viruses was established, which, additionally, enabled species identification of the detected viruses by subsequent sequencing.

Article Title: Divergent expression patterns of miR164 and CUP-SHAPED COTYLEDON genes in palms and other monocots: implication for the evolution of meristem function in angiosperms.
Article Snippet: The PCR products were cloned using a pGEM-T easy ligation kit (Promega, France) with Escherichia coli JM109 competent cells (Promega) and sequenced by Beckman Coulter Genomics (UK).

Article Title: Genetic mutations in sodium channel domain II and carboxylesterase genes associated with phenotypic resistance against synthetic pyrethroids by Rhipicephalus (Boophilus) decoloratus ticks in Uganda.
Article Snippet: Please cite this article as: Patrick Vudriko, Rika Umemiya-Shirafuji, James Okwee-Acai, Dickson Stuart Tayebwa, Joseph Byaruhanga, Charoonluk Jirapattharasate, Mingming Liu, Paul Franck Adjou Moumouni, Kozo Fujisaki, Xuenan Xuan, Hiroshi Suzuki , Genetic mutations in sodium channel domain II and carboxylesterase genes associated with phenotypic resistance against synthetic pyrethroids by Rhipicephalus (Boophilus) decoloratus ticks in Uganda, Pesticide Biochemistry and Physiology (2017), doi: 10.1016/ j.pestbp.2017.07.009

Article Title: C190A knockdown mutation in sodium channel domain II of pyrethroid-resistant Rhipicephalus appendiculatus.
Article Snippet: This study investigated the target site mutations in the partial sequence of voltage-sensitive sodium channel (VSSC) domain II of synthetic pyrethroid (SP)-resistant Rhipicephalus appendiculatus.. Genomic DNA was extracted from seven tick populations (two susceptible and five resistant) collected from central, eastern and southwestern Uganda.. The PCR amplicons of the VSSC domain II were cloned and sequenced to determine novel single nucleotide polymorphisms (SNP).

Article Title: Conserved miR164-targeted NAC genes negatively regulate drought resistance in rice
Article Snippet: PCR products were cloned using the pGEM-T easy ligation kit (Promega) with Escherichia coli Top10 competent cells (Invitrogen) and sequenced.

Article Title: Sequence conservation and divergence in miR164C1 and its target, CUC1, in Brassica species
Article Snippet: An emerging aspect of plant development and adaptation studies is microRNA (miRNA)-mediated gene regulation and to understand post-transcriptional gene silencing (PTGS) dynamics.. Studies performed within an evolutionary context, such as specific plant lineages and genome status (e.g. ploidy level) can highlight the selection pressure and constraints guiding the evolutionary trajectory of regulatory modules.. We have investigated conservation and allelic diversity in one such module composed of CUC1 (CUP-SHAPED COTYLEDON 1), a NAC domain family member, and miR164C, within Brassica species.

Article Title: Systems and methods to assess microbiomes and treatments thereof
Article Snippet: One to three microliters of the 20 μl purified product was ligated into pGEM-T Easy vector using the pGEM-T Easy Ligation Kit (Promega) according to the manufacturer's specifications.

DNA Purification:

Article Title: Development and validation of a universal S-segment-based real-time RT-PCR assay for the detection of Simbu serogroup viruses.
Article Snippet: Simbu serogroup viruses induce acute clinical diseases and abnormal courses of pregnancies in livestock.. In Israel, two members of this serogroup, namely Akabane virus (AKAV) and Shuni virus (SHUV), were recently detected and, in Europe, Schmallenberg virus (SBV) poses a threat to ruminants.. To address this emerging problem, a universal S-segment-based real-time RT-PCR assay (Uni-S) for the detection of Simbu serogroup viruses was established, which, additionally, enabled species identification of the detected viruses by subsequent sequencing.

Article Title: Divergent expression patterns of miR164 and CUP-SHAPED COTYLEDON genes in palms and other monocots: implication for the evolution of meristem function in angiosperms.
Article Snippet: The PCR products were cloned using a pGEM-T easy ligation kit (Promega, France) with Escherichia coli JM109 competent cells (Promega) and sequenced by Beckman Coulter Genomics (UK).

Article Title: Genetic mutations in sodium channel domain II and carboxylesterase genes associated with phenotypic resistance against synthetic pyrethroids by Rhipicephalus (Boophilus) decoloratus ticks in Uganda.
Article Snippet: Please cite this article as: Patrick Vudriko, Rika Umemiya-Shirafuji, James Okwee-Acai, Dickson Stuart Tayebwa, Joseph Byaruhanga, Charoonluk Jirapattharasate, Mingming Liu, Paul Franck Adjou Moumouni, Kozo Fujisaki, Xuenan Xuan, Hiroshi Suzuki , Genetic mutations in sodium channel domain II and carboxylesterase genes associated with phenotypic resistance against synthetic pyrethroids by Rhipicephalus (Boophilus) decoloratus ticks in Uganda, Pesticide Biochemistry and Physiology (2017), doi: 10.1016/ j.pestbp.2017.07.009

Article Title: C190A knockdown mutation in sodium channel domain II of pyrethroid-resistant Rhipicephalus appendiculatus.
Article Snippet: This study investigated the target site mutations in the partial sequence of voltage-sensitive sodium channel (VSSC) domain II of synthetic pyrethroid (SP)-resistant Rhipicephalus appendiculatus.. Genomic DNA was extracted from seven tick populations (two susceptible and five resistant) collected from central, eastern and southwestern Uganda.. The PCR amplicons of the VSSC domain II were cloned and sequenced to determine novel single nucleotide polymorphisms (SNP).

Article Title: Conserved miR164-targeted NAC genes negatively regulate drought resistance in rice
Article Snippet: PCR products were cloned using the pGEM-T easy ligation kit (Promega) with Escherichia coli Top10 competent cells (Invitrogen) and sequenced.

Article Title: Sequence conservation and divergence in miR164C1 and its target, CUC1, in Brassica species
Article Snippet: An emerging aspect of plant development and adaptation studies is microRNA (miRNA)-mediated gene regulation and to understand post-transcriptional gene silencing (PTGS) dynamics.. Studies performed within an evolutionary context, such as specific plant lineages and genome status (e.g. ploidy level) can highlight the selection pressure and constraints guiding the evolutionary trajectory of regulatory modules.. We have investigated conservation and allelic diversity in one such module composed of CUC1 (CUP-SHAPED COTYLEDON 1), a NAC domain family member, and miR164C, within Brassica species.

Article Title: Systems and methods to assess microbiomes and treatments thereof
Article Snippet: One to three microliters of the 20 μl purified product was ligated into pGEM-T Easy vector using the pGEM-T Easy Ligation Kit (Promega) according to the manufacturer's specifications.

Clone Assay:

Article Title: Development and validation of a universal S-segment-based real-time RT-PCR assay for the detection of Simbu serogroup viruses.
Article Snippet: Simbu serogroup viruses induce acute clinical diseases and abnormal courses of pregnancies in livestock.. In Israel, two members of this serogroup, namely Akabane virus (AKAV) and Shuni virus (SHUV), were recently detected and, in Europe, Schmallenberg virus (SBV) poses a threat to ruminants.. To address this emerging problem, a universal S-segment-based real-time RT-PCR assay (Uni-S) for the detection of Simbu serogroup viruses was established, which, additionally, enabled species identification of the detected viruses by subsequent sequencing.

Article Title: Divergent expression patterns of miR164 and CUP-SHAPED COTYLEDON genes in palms and other monocots: implication for the evolution of meristem function in angiosperms.
Article Snippet: The PCR products were cloned using a pGEM-T easy ligation kit (Promega, France) with Escherichia coli JM109 competent cells (Promega) and sequenced by Beckman Coulter Genomics (UK).

Article Title: Genetic mutations in sodium channel domain II and carboxylesterase genes associated with phenotypic resistance against synthetic pyrethroids by Rhipicephalus (Boophilus) decoloratus ticks in Uganda.
Article Snippet: Please cite this article as: Patrick Vudriko, Rika Umemiya-Shirafuji, James Okwee-Acai, Dickson Stuart Tayebwa, Joseph Byaruhanga, Charoonluk Jirapattharasate, Mingming Liu, Paul Franck Adjou Moumouni, Kozo Fujisaki, Xuenan Xuan, Hiroshi Suzuki , Genetic mutations in sodium channel domain II and carboxylesterase genes associated with phenotypic resistance against synthetic pyrethroids by Rhipicephalus (Boophilus) decoloratus ticks in Uganda, Pesticide Biochemistry and Physiology (2017), doi: 10.1016/ j.pestbp.2017.07.009

Article Title: C190A knockdown mutation in sodium channel domain II of pyrethroid-resistant Rhipicephalus appendiculatus.
Article Snippet: This study investigated the target site mutations in the partial sequence of voltage-sensitive sodium channel (VSSC) domain II of synthetic pyrethroid (SP)-resistant Rhipicephalus appendiculatus.. Genomic DNA was extracted from seven tick populations (two susceptible and five resistant) collected from central, eastern and southwestern Uganda.. The PCR amplicons of the VSSC domain II were cloned and sequenced to determine novel single nucleotide polymorphisms (SNP).

Article Title: Conserved miR164-targeted NAC genes negatively regulate drought resistance in rice
Article Snippet: PCR products were cloned using the pGEM-T easy ligation kit (Promega) with Escherichia coli Top10 competent cells (Invitrogen) and sequenced.

Article Title: Sequence conservation and divergence in miR164C1 and its target, CUC1, in Brassica species
Article Snippet: An emerging aspect of plant development and adaptation studies is microRNA (miRNA)-mediated gene regulation and to understand post-transcriptional gene silencing (PTGS) dynamics.. Studies performed within an evolutionary context, such as specific plant lineages and genome status (e.g. ploidy level) can highlight the selection pressure and constraints guiding the evolutionary trajectory of regulatory modules.. We have investigated conservation and allelic diversity in one such module composed of CUC1 (CUP-SHAPED COTYLEDON 1), a NAC domain family member, and miR164C, within Brassica species.

Article Title: Systems and methods to assess microbiomes and treatments thereof
Article Snippet: One to three microliters of the 20 μl purified product was ligated into pGEM-T Easy vector using the pGEM-T Easy Ligation Kit (Promega) according to the manufacturer's specifications.

Transformation Assay:

Article Title: Development and validation of a universal S-segment-based real-time RT-PCR assay for the detection of Simbu serogroup viruses.
Article Snippet: Simbu serogroup viruses induce acute clinical diseases and abnormal courses of pregnancies in livestock.. In Israel, two members of this serogroup, namely Akabane virus (AKAV) and Shuni virus (SHUV), were recently detected and, in Europe, Schmallenberg virus (SBV) poses a threat to ruminants.. To address this emerging problem, a universal S-segment-based real-time RT-PCR assay (Uni-S) for the detection of Simbu serogroup viruses was established, which, additionally, enabled species identification of the detected viruses by subsequent sequencing.

Article Title: Divergent expression patterns of miR164 and CUP-SHAPED COTYLEDON genes in palms and other monocots: implication for the evolution of meristem function in angiosperms.
Article Snippet: The PCR products were cloned using a pGEM-T easy ligation kit (Promega, France) with Escherichia coli JM109 competent cells (Promega) and sequenced by Beckman Coulter Genomics (UK).

Article Title: Genetic mutations in sodium channel domain II and carboxylesterase genes associated with phenotypic resistance against synthetic pyrethroids by Rhipicephalus (Boophilus) decoloratus ticks in Uganda.
Article Snippet: Please cite this article as: Patrick Vudriko, Rika Umemiya-Shirafuji, James Okwee-Acai, Dickson Stuart Tayebwa, Joseph Byaruhanga, Charoonluk Jirapattharasate, Mingming Liu, Paul Franck Adjou Moumouni, Kozo Fujisaki, Xuenan Xuan, Hiroshi Suzuki , Genetic mutations in sodium channel domain II and carboxylesterase genes associated with phenotypic resistance against synthetic pyrethroids by Rhipicephalus (Boophilus) decoloratus ticks in Uganda, Pesticide Biochemistry and Physiology (2017), doi: 10.1016/ j.pestbp.2017.07.009

Article Title: C190A knockdown mutation in sodium channel domain II of pyrethroid-resistant Rhipicephalus appendiculatus.
Article Snippet: This study investigated the target site mutations in the partial sequence of voltage-sensitive sodium channel (VSSC) domain II of synthetic pyrethroid (SP)-resistant Rhipicephalus appendiculatus.. Genomic DNA was extracted from seven tick populations (two susceptible and five resistant) collected from central, eastern and southwestern Uganda.. The PCR amplicons of the VSSC domain II were cloned and sequenced to determine novel single nucleotide polymorphisms (SNP).

Article Title: Conserved miR164-targeted NAC genes negatively regulate drought resistance in rice
Article Snippet: PCR products were cloned using the pGEM-T easy ligation kit (Promega) with Escherichia coli Top10 competent cells (Invitrogen) and sequenced.

Article Title: Sequence conservation and divergence in miR164C1 and its target, CUC1, in Brassica species
Article Snippet: An emerging aspect of plant development and adaptation studies is microRNA (miRNA)-mediated gene regulation and to understand post-transcriptional gene silencing (PTGS) dynamics.. Studies performed within an evolutionary context, such as specific plant lineages and genome status (e.g. ploidy level) can highlight the selection pressure and constraints guiding the evolutionary trajectory of regulatory modules.. We have investigated conservation and allelic diversity in one such module composed of CUC1 (CUP-SHAPED COTYLEDON 1), a NAC domain family member, and miR164C, within Brassica species.

Article Title: Systems and methods to assess microbiomes and treatments thereof
Article Snippet: One to three microliters of the 20 μl purified product was ligated into pGEM-T Easy vector using the pGEM-T Easy Ligation Kit (Promega) according to the manufacturer's specifications.



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